biG 10 - Interspecies reactive Mab to CD14 (pork, bovine, horse, dog) aa.9-13, 39-44, IgG1

Category

Mouse monoclonal antibody protein G purified (lyophilized)

Reconstitution

reconstitute with 100 µl distilled water

Isotype

IgG₁

Antigen

Human monocytes, monocytes of different species and soluble human CD14

Special note

biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Characteristic

inhibits binding of LPS to CD14, binding epitope: amino acid 9-13, 39-44, total inhibition of LPS-binding to CD14: 100µg/ml
Binding titre at human CD14 transfected CHO-cells: more than 10.000

Application

Suitable for immunostaining of CD14 positive cells (Flow cytometry)

ELISA, CD14 inhibition studies, Western Blot

Storage

Long time storage at –20°C or –80°C

Quantity

100 µg in PBS without NaN₃ or other stabilisation before lyophilisation

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Material Safety Data Sheet - Proteins and Antibodies