Category
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Protein G purified, lyophilized
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Reconstitution
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For reconstitution add 100µl water to get concentration of 1 mg/ml in PBS
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Isotype
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IgG1
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Antigen
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Monocytes of different species and immunoaffinity purified soluble human CD14
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Special note
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biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most mono¬cytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. Repeated freezing and thawing should be avoided.
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Characteristic
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20 µg/ml inhibits binding of 0.5 µg/ml LPS to CD14, binding epitope amino acid 9-13, 39-44, cross reacting with CD14 of cattle++, swine ++, dog+ horse+ Binding titre at human CD14 transfected CHO-cells: more than 10.000
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Application
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Suitable for immunostaining of CD14 positive cells (flow cytometry) ELISA, CD14 inhibition studies, Western Blot
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Storage
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Long time storage at –20°C or –80°C
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Quantity
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100 µg in PBS without NaN3 or other stabilisation before lyophilisation
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IgG-content |
1 mg/ml PBS |
Download
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Material Safety Data Sheet - Proteins and Antibodies
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