|
Category
|
Protein G purified, lyophilized
|
|
Reconstitution
|
For reconstitution add 100µl water to get concentration of 1 mg/ml in PBS
|
|
Isotype
|
IgG1
|
|
Antigen
|
Monocytes of different species and immunoaffinity purified soluble human CD14
|
|
Special note
|
biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most mono¬cytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin. Repeated freezing and thawing should be avoided.
|
|
Characteristic
|
20 µg/ml inhibits binding of 0.5 µg/ml LPS to CD14, binding epitope amino acid 9-13, 39-44, cross reacting with CD14 of cattle++, swine ++, dog+ horse+
Binding titre at human CD14 transfected CHO-cells: more than 10.000
|
|
Application
|
Suitable for immunostaining of CD14 positive cells (flow cytometry) ELISA, CD14 inhibition studies, Western Blot
|
|
Storage
|
Long time storage at –20°C or –80°C
|
|
Quantity
|
100 µg in PBS without NaN3 or other stabilisation before lyophilisation
|
| IgG-content |
1 mg/ml PBS |
|
Download
|
Material Safety Data Sheet - Proteins and Antibodies
|
