Recombinant human CD14 purified by affinity chromatography
Product information "Recombinant human CD14 purified by affinity chromatography"
Characteristic |
The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. |
hCD14-content |
1.0 mg/ml phosphate-buffered saline, pH 7.2 determined by hCD14 ELISA with defined standard. -lyophilized- For reconstitution add 10 µl of water to each tube |
Purification |
affinity chromatography with monoclonal antibody to hCD14 biG2 and controlled by SDS page (90-95% purity) |
Endotoxin content |
Less than 0.01 ng/ml (LAL-test „Chromogenix) |
Special note |
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein. Up to 10 µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6 x 105 CD14+CHO transfectants (FACS) |
Quantity |
10 µg |
Storage |
long time storage at –80°C |
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