Recombinant human CD14 purified by affinity chromatography

Characteristic

The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

hCD14-content

1.0 mg/ml phosphate-buffered saline, pH 7.2 determined by hCD14 ELISA with defined standard. -lyophilized-

For reconstitution add 10 µl of water to each tube

Purification

affinity chromatography with monoclonal antibody to hCD14 biG2 and controlled by SDS page (90-95% purity)

Endotoxin content

Less than 0.01 ng/ml (LAL-test „Chromogenix)
after heating (5', 75°C)

Special note

The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.

Up to 10 µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6 x 10⁵ CD14⁺CHO transfectants (FACS)

Quantity

10 µg

Storage

long time storage at –80°C

Download

Material Safety Data Sheet - Proteins and Antibodies