Recombinant human LBP
Product information "Recombinant human LBP"
Characteristic |
The LBP is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. |
huLBP-content |
0.25 mg/ml phosphate-buffered saline, pH 7.2 (determined by huLBP-ELISA with defined standard) |
Purification |
By his-tag with metal affinity purification with Talon and controlled by SDS page (purity:90-95%). |
Endotoxin content |
Less than 0.01ng/ml (determined by LAL-ELISA-test „Chromogenix“ after heating 5' 75°C) |
Special note |
Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS). |
Quantity |
10 µg, lyophilized |
Storage |
long time: -80°C. Short time storage is possible at 4°C or -20°C |
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